STUDI IN SILICO SINGLE CHAIN VARIABLE FRAGMENT (SCFV) SELEKTIF TERHADAP HORMON BASIC NATRIURETIC PEPTIDE (BNP)

Fragment (scFv) Specific for Intercellular Lactobacilli-Expressed Single-Chain Variable

Isolation of single-chain variable fragment (scFv) antibodies against synthetic peptide fragments of human cathepsin S.

This transaction reports on the on-going attempt to isolate anti-peptide scFv antibodies from an scFv phage-display library capable of detecting the native protein from which the peptide sequence was derived. The display of antibody fragments on the surface of filamentous bacteriophage by fusion to the minor coat protein (plll) and selection of phage with antigen, has provided a powerful method...

RECOMBINANT SINGLE CHAIN VARIABLE FRAGMENT ANTIBODIES (scFv) AGAINST Pro144-Leu155 FRAGMENT OF HUMAN PROTEIN C.

The aim of this work was to obtain the recombinant single chain variable fragments of antibodies (scFv) against human protein C, the key component of blood anticoagulation system. For this purpose a peptide that mimics a Pro144-Leu155 sequence of protein C was synthesized and the murine immune scFv library against this peptide was constructed. The protein C specific scFv 9E were selected from t...

Isolation of single chain variable fragment (scFv) specific for Cry1C toxin from human single fold scFv libraries.

As bioinsecticides Bacillus thuringiensis Cry1C δ-endotoxins also have been used in genetically modified crops worldwide since last century. In this study, single chain variable fragments (scFvs), which could specifically recognize and detect Cry1C in food samples, were isolated from naive phage displayed human antibody libraries (Tomlinson I + J) by iterative affinity selection procedure inste...

Generation of a Single Chain Antibody Variable Fragment (scFv) to Sense Selectively RhoB Activation

Determining the cellular level of activated form of RhoGTPases is of key importance to understand their regulatory functions in cell physiopathology. We previously reported scFvC1, that selectively bind to the GTP-bound form of RhoA, RhoB and RhoC. In this present study we generate, by molecular evolution, a new phage library to isolate scFvs displaying high affinity and selectivity to RhoA and...